Opal and Aspen would have had a rough life even if they would have tested negative and had lived. As much as the Mama tried, the fuzzballs did not want to learn that humans are good. They were clearly feral and would have been returned to their colony. While the colony they came from is well taken care of, it is a much harder life living on the streets.
We are sad that Opal and Aspen are no longer with us but we hope they know someone cared for them. We hope they will meet us at the Rainbow Bridge someday.